CD62L/L-Selectin (MEL-14) Rat mAb (PE-Cy7® Conjugate) #61885
Filter:
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2a kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PE-Cy7® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.06μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:300 |
| Flow Cytometry (Live) | 1:300 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD62L/L-Selectin (MEL-14) Rat mAb (PE-Cy7® Conjugate) recognizes endogenous levels of total mouse CD62L protein.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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