CD57 (HNK-1) Mouse mAb (Pacific Blue™ Conjugate) #25978
Filter:
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgM |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody CD57 (HNK-1) Mouse mAb #72031.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
| Flow Cytometry (Live) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. This product is stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD57 (HNK-1) Mouse mAb (Pacific Blue™ Conjugate) recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells.
Background
CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1).
- Focosi, D. et al. (2010) J Leukoc Biol 87, 107-16.
- Górska, A. et al. (2016) Arch Immunol Ther Exp (Warsz) 64, 497-503.
- Kared, H. et al. (2016) Cancer Immunol Immunother 65, 441-52.
- Lopez-Vergès, S. et al. (2010) Blood 116, 3865-74.
- Mechtersheimer, G. et al. (1991) Cancer Res 51, 1300-7.
- Stelin, S. et al. (2009) J Indian Soc Periodontol 13, 150-4.
- Künemund, V. et al. (1988) J Cell Biol 106, 213-23.
- Sze, D.M. et al. (2001) Blood 98, 2817-27.
- Nielsen, C.M. et al. (2013) Front Immunol 4, 422.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Pacific Blue is a trademark of Life Technologies Corporation.
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