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CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (HRP Conjugate) #59818
Filter:
- WB
Western blot analysis of extracts from 32D cells (CD31/PECAM-1 positive) and 3T3 cells (CD31/PECAM-1 negative) using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (HRP Conjugate) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
To Purchase # 59818
Cat. #
Size
Price
Inventory
59818S
100 µl
¥5,460
无库存
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb #77699.
| MW (kDa) | 135 |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total CD31 (PECAM-1) protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala451 of mouse CD31 (PECAM-1) protein.
Background
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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