CD24 (M1/69) Rat mAb (violetFluor™ 450 Conjugate) #9705
Filter:
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to violetFluor™ 450 and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:40 |
| Flow Cytometry (Live) | 1:40 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD24 (M1/69) Rat mAb (violetFluor™ 450 Conjugate) recognizes endogenous levels of total CD24 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development (1-3). It also binds to Siglec-10 in humans or Siglec-G in mice (4,5). CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types (6,7).
- Sammar, M. et al. (1994) Int Immunol 6, 1027-36.
- Aigner, S. et al. (1997) Blood 89, 3385-95.
- Fang, X. et al. (2010) Cell Mol Immunol 7, 100-3.
- Chen, G.Y. et al. (2009) Science 323, 1722-5.
- Toubai, T. et al. (2014) Blood 123, 3512-23.
- Jaggupilli, A. and Elkord, E. (2012) Clin Dev Immunol 2012, 708036.
- Yu, Z. et al. (2012) Int J Biochem Cell Biol 44, 2144-51.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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