CD20 (2H7) Mouse mAb (APC Conjugate) #69077
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Flow cytometric analysis of live human peripheral blood mononuclear cells using CD20 (2H7) Mouse mAb (APC Conjugate) (solid line) compared to concentration-matched Mouse Isotype Control (APC Conjugate) (dashed line).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in human cells.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD20 (2H7) Mouse mAb (APC Conjugate) recognizes endogenous levels of total CD20 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6).
The 2H7 antibody is widely used to identify both normal and malignant B cells (7).
The 2H7 antibody is widely used to identify both normal and malignant B cells (7).
- Stashenko, P. et al. (1980) J Immunol 125, 1678-85.
- Tedder, T.F. et al. (1985) J Immunol 135, 973-9.
- Tedder, T.F. et al. (1988) J Immunol 141, 4388-94.
- Bubien, J.K. et al. (1993) J Cell Biol 121, 1121-32.
- Li, H. et al. (2003) J Biol Chem 278, 42427-34.
- Uchida, J. et al. (2004) J Exp Med 199, 1659-69.
- Liu, A.Y. et al. (1987) J Immunol 139, 3521-6.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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