CD19 (SJ25C1) Mouse mAb (PerCP Conjugate) #47432
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Inquiry Info. # 47432
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PerCP and tested in-house for direct flow cytometry analysis in human cells.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:20 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD19 (SJ25C1) Mouse mAb (PerCP Conjugate) recognizes endogenous levels of total CD19 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).
The SJ25C1 antibody is widely used to identify cells of the B-lymphocyte lineage (6).
The SJ25C1 antibody is widely used to identify cells of the B-lymphocyte lineage (6).
- Tedder, T.F. et al. (1997) Immunity 6, 107-18.
- Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
- Fujimoto, M. et al. (2000) Immunity 13, 47-57.
- O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
- Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.
- Bejcek, B.E. et al. (1995) Cancer Res 55, 2346-51.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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