CD11c (N418) Hamster mAb (PerCP-Cy5.5® Conjugate) #93983
Filter:
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Hamster (Armenian) IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:80 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD11c (N418) Hamster mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9).
The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9).
- Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9.
- Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
- Siegal, F.P. et al. (1999) Science 284, 1835-7.
- Racine, R. et al. (2008) J Immunol 181, 1375-85.
- Werfel, T. et al. (1991) J Immunol 147, 2423-7.
- Cabañas, C. et al. (1988) Hybridoma 7, 167-76.
- Kristensen, J.S. et al. (1987) Blood 70, 1063-8.
- Finkelman, F.D. et al. (1996) J Immunol 157, 1406-14.
- Sadhu, C. et al. (2007) J Leukoc Biol 81, 1395-403.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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