CD11b/ITGAM (M1/70) Rat mAb (FITC Conjugate) #24442
Filter:
- F
Flow cytometric analysis of live mouse bone marrow cells using CD11b/ITGAM (M1/70) Rat mAb (FITC Conjugate) (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (FITC Conjugate) #56722 (dashed line).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
| Flow Cytometry (Live) | 1:100 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD11b/ITGAM (M1/70) Rat mAb (FITC Conjugate) recognizes endogenous levels of total CD11b protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human, Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).
The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).
The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).
- Solovjov, D.A. et al. (2005) J Biol Chem 280, 1336-45.
- Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
- Fagerholm, S.C. et al. (2006) Blood 108, 3379-86.
- Rosetti, F. and Mayadas, T.N. (2016) Immunol Rev 269, 175-93.
- Finkelman, F.D. et al. (1996) J Immunol 157, 1406-14.
- Holmberg, L.A. et al. (1981) J Immunol 127, 1792-9.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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