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B7-H3 (D9M2L) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #65831
Filter:
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated B7-H3 (D9M2L) XP® Rabbit mAb #14058.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
B7-H3 (D9M2L) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total B7-H3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala94 of human B7-H3 protein.
Background
B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8).
- Chapoval, A.I. et al. (2001) Nat Immunol 2, 269-74.
- Leitner, J. et al. (2009) Eur J Immunol 39, 1754-64.
- Ling, V. et al. (2003) Genomics 82, 365-77.
- Petroff, M.G. et al. (2005) Am J Pathol 167, 465-73.
- Roth, T.J. et al. (2007) Cancer Res 67, 7893-900.
- Loo, D. et al. (2012) Clin Cancer Res 18, 3834-45.
- Seaman, S. et al. (2007) Cancer Cell 11, 539-54.
- Zang, X. et al. (2010) Mod Pathol 23, 1104-12.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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