β3-Tubulin (E9F3E) Mouse mAb (Alexa Fluor® 647 Conjugate) #82087
Filter:
- IF
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum, labeled with β3-Tubulin (E9F3E) Mouse mAb (Alexa Fluor® 647 Conjugate) (left, red) and co-labeled with Tau (D1M9X) XP® Rabbit mAb #46687 (right, green) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated β3-Tubulin (E9F3E) Mouse mAb #45058.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
β3-Tubulin (E9F3E) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total β3-tubulin protein. This antibody conjugate does not cross-react with tubulin isoforms expressed in non-neuronal cells.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β3-tubulin protein.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5).
β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5).
- Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
- Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
- Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
- Tischfield, M.A. et al. (2010) Cell 140, 74-87.
- Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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