Render Target: SSR
Render Timestamp: 2025-01-09T23:34:33.961Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-20 06:15:07.770
Product last modified at: 2025-01-01T09:06:23.052Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

β-Catenin (D10A8) XP® Rabbit mAb (Biotinylated) #13727

Filter:
  • WB
Western Blotting Image 1: β-Catenin (D10A8) XP® Rabbit mAb (Biotinylated)
Western blot analysis of extracts from 3T3 and HeLa cells using β-Catenin (D10A8) XP® Rabbit mAb (Biotinylated).

To Purchase # 13727S

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 92
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Catenin (D10A8) XP® Rabbit mAb #8480.
MW (kDa) 92

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

β-Catenin (D10A8) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total β-catenin protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Zebrafish, Bovine, Pig, Horse, Guinea Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.