R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
AML1 (D33G6) XP® Rabbit mAb (PE Conjugate) #15002
Filter:
- F
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AML1 (D33G6) XP® Rabbit mAb #4336.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
AML1 (D33G6) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total AML1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino terminus of human AML1 protein.
Background
AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
- Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39.
- Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63.
- Okuda, T. et al. (1996) Cell 84, 321-30.
- Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9.
- North, T.E. et al. (2004) Stem Cells 22, 158-68.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
- Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
- Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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