R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Aiolos (D1C1E) Rabbit mAb (Pacific Blue™ Conjugate) #40386
Filter:
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Aiolos (D1C1E) Rabbit mAb #15103.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Aiolos (D1C1E) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of total Aiolos protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein.
Background
Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). Ikaros family proteins control lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, and elevated levels of IgG and IgE. In addition, Aiolos null mice develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Aberrant expression of Aiolos in transformed epithelial cells promotes anchorage independence through downregulation of adhesion-related genes (6). Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (7-9).
- Morgan, B. et al. (1997) EMBO J 16, 2004-13.
- Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
- Kim, J. et al. (1999) Immunity 10, 345-55.
- Wang, J.H. et al. (1998) Immunity 9, 543-53.
- Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
- Li, X. et al. (2014) Cancer Cell 25, 575-89.
- Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
- Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
- Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Pacific Blue is a trademark of Life Technologies Corporation.
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