Product Information

Product Usage Information

Vinblastine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 550 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	909.05 g/mol
Purity	>98%
Molecular Formula	C₄₆H₅₈N₄O₉•H₂SO₄
CAS	143-67-9
Solubility	Soluble in DMSO at 50mg/ml and H2O at 10mg/ml.

Background

Vinblastine is a vinca alkaloid derived from the plant Catharanthus roseus. Like other vinca alkaloids, it acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Investigators have demonstrated that vinblastine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (K_i = ~178 nM) (1). This disruption of mitotic spindle causes mitotic arrest, inhibits cell proliferation, and induces apoptosis (1-3). Studies have suggested that high concentrations of vinblastine depolymerize microtubules, but this is not required for inhibition of cell proliferation (2). Treatment of cells with vinblastine has been shown to activate the SAPK/JNK pathway, leading to expression and phosphorylation of c-Jun (3,4) and phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-xL (5,6).

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Vinblastine #14255