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Product last modified at: 2024-10-17T19:15:08.681Z
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TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174

    Product Information

    Product Usage Information

    TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

    TPA is soluble in DMSO and ethanol.

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 616.83 g/mol
    Purity >99.5%
    Molecular Formula C36H56O8
    CAS 16561-29-8
    Solubility Soluble in DMSO and EtOH at 25mg/ml.

    Background

    TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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