Concanamycin A #29442
Product Information
Product Usage Information
Concanamycin A is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 10 μg of powder in 1.15 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Product Description
| Molecular Weight | 866.1 g/mol |
| Purity | >98% |
| Molecular Formula | C46H75NO14 |
| CAS | 80890-47-7 |
| Solubility | Soluble in DMSO at 50 mg/mL. |
Background
Concanamycin A is a macrolide antibiotic derived from S. diastatochromogenes that is effective against several fungi and yeasts (1). Concanamycin A is an exceptionally potent and specific inhibitor of the ATP-driven proton pumps known as vacuolar type H+-ATPases (V-ATPases). V-ATPases acidify intracellular compartments and translocate protons across the plasma membrane. Intracellular V-ATPases play an important role in endocytosis and intracellular membrane trafficking, while plasma membrane V-ATPases are important in processes such as urinary acidification and bone resorption (2). Treatment of murine cells with Concanamycin A results in apoptosis, evidenced by an increase in fragmented DNA and the number of apoptotic cells with hypodiploid DNA (3). Concanamycin A induced production of nitric oxide and decreased cell growth and survival in mouse leukemic monocyte cells (4). Concanamycin A reversed the downregulation of cell surface MHC-I by the HIV-encoded accessory protein Nef, suggesting a possible therapeutic role of Concanamycin A in enhancing the immune-mediated clearance of HIV-infected cells (5).
- Kinashi, H. et al. (1984) J Antibiot (Tokyo) 37, 1333-43.
- Jefferies, K.C. et al. (2008) Arch Biochem Biophys 476, 33-42.
- Nishihara, T. et al. (1995) Biochem Biophys Res Commun 212, 255-62.
- Hong, J. et al. (2006) J Pharmacol Exp Ther 319, 672-81.
- Painter, M.M. et al. (2020) Proc Natl Acad Sci U S A 117, 23835-23846.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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