VGLUT1 (E9D2B) Mouse mAb #98199
Filter:
- WB
- IF
Western blot analysis of extracts from human, mouse, and rat brain tissues using VGLUT1 (E9D2B) Mouse mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Mouse IgG2b kappa |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:800 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #34007.
For a carrier free (BSA and azide free) version of this product see product #34007.
Protocol
Specificity / Sensitivity
VGLUT1 (E9D2B) Mouse mAb recognizes endogenous levels of total VGLUT1 protein. This antibody does not cross-react with other VGLUT proteins. This antibody detects a 45 kDa band of unknown origin in human cerebellum lysate.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human VGLUT1 protein.
Background
Glutamatergic neurons release glutamate, the most common excitatory neurotransmitter. Their synaptic vesicles are filled with glutamate by vesicular glutamate transporters, VGLUTs (1). VGLUT1, also called solute carrier family 17 member 7 (SLC17A7), was first identified as an inorganic phosphate transporter (2). Despite the absence of homology with neurotransmitter transporters, VGLUT1 was later demonstrated to be a glutamate transporter (1) specific to glutamatergic neurons (3). Closely related to VGLUT1, VGLUT2 and VGLUT3 are also involved in glutamate uptake into synaptic vesicles, but define different neuronal subpopulations (4,5). VGLUT1 and VGLUT2 are the most abundant isoforms. VGLUT1 is expressed in the cortex, hippocampus, and cerebellar cortex, while VGLUT2 is mostly found in the thalamus (6,7). VGLUT3 is expressed in hair cells of the auditory system (8).
- Bellocchio, E.E. et al. (2000) Science 289, 957-60.
- Ni, B. et al. (1996) J Neurochem 66, 2227-38.
- Takamori, S. et al. (2000) Nature 407, 189-94.
- Fremeau, R.T. et al. (2001) Neuron 31, 247-60.
- Fremeau, R.T. et al. (2002) Proc Natl Acad Sci U S A 99, 14488-93.
- Herzog, E. et al. (2001) J Neurosci 21, RC181.
- Kaneko, T. and Fujiyama, F. (2002) Neurosci Res 42, 243-50.
- Seal, R.P. et al. (2008) Neuron 57, 263-75.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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