R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD62L/L-Selectin (E7X9I) Rabbit mAb (BSA and Azide Free) #98131
Filter:
- WB
- IHC
Western blot analysis of extracts from various cell lines using CD62L/L-Selectin (E7X9I) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CD62L/L-Selectin protein in HeLa cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60-100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
This product is the carrier free version of product #58225. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #58225
For standard formulation of this product see product #58225
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CD62L/L-Selectin (E7X9I) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD62L/L-Selectin protein. Non-specific staining was observed in the surface epithelium of tonsil by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CD62L/L-Selectin recombinant protein.
Background
L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).
Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7).
Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7).
- Gallatin, W.M. et al. Nature 304, 30-4.
- Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
- Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
- Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
- Finger, E.B. et al. (1996) Nature 379, 266-9.
- Sallusto, F. et al. (1999) Nature 401, 708-12.
- Ivetic, A. et al. (2019) Front Immunol 10, 1068.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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