Cleaved DFF45 (Asp224) Antibody #9731
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. The antibody does not recognize full length DFF45 or other cleavage products of DFF45.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Enari, M. et al. (1998) Nature 391, 43-50.
- Sakahira, H. et al. (1998) Nature 391, 96-99.
- Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
- Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
- Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
- Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
- Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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