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Rb (4H1) Mouse mAb

Rb (4H1) Mouse mAb #9309

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WB
IP
IHC
IF
F
ChIP

Supporting Data

REACTIVITY	H Mk B Pg
SENSITIVITY	Endogenous
MW (kDa)	110
Source/Isotype	Mouse IgG2a

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry
ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
Mk-Monkey
B-Bovine
Pg-Pig

Product Information

Product Usage Information

For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:2000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:800 - 1:3200
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200
Flow Cytometry (Fixed/Permeabilized)	1:3200
Chromatin IP	1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #61121.

Protocol

Specificity / Sensitivity

Rb (4H1) Mouse mAb detects endogenous levels of total Rb protein. The antibody does not cross-react with the Rb homologues p107 or p130, or with other proteins.

Species Reactivity:

Human, Monkey, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a Rb-C fusion protein containing residues 701-928 of human Rb. The epitope corresponds to a region surrounding His890 of human Rb.

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

Database Links

UniProt ID: P06400

Entrez-Gene Id: 5925

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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