Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231
Filter:
- WB
Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using Phospho-MKK3/MKK6 (Ser189/207) Antibody (upper) or MKK3 Antibody #9232 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 Phospho-MKK3. 41 Phospho-MKK6. |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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