PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit #8803
Representative motif analysis of 2362 phospho-tyrosine tryptic peptides generated from a PhosphoScan® experiment of Jurkat cells treated with Calyculin A #9902 and pervanadate using PTMScan® Phospho-Tyrosine Motif (Y*) (P-Tyr-1000) Immunoaffinity Beads. Motif logo was generated using the PhosphoSitePlus® Logo Generator and reflects relative frequency of residues in each position. For more information, please visit PhosphoSitePlus® at www.phosphosite.org.
To Purchase # 8803
Additional Information
This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.
- Product Includes
- Related Products
| Product Includes | Volume (with Count) |
|---|---|
| PTMScan® Phospho-Tyrosine Motif (Y*) (P-Tyr-1000) Immunoaffinity Beads #8876 | 10 x 80 µl |
| PTMScan® IAP Buffer (10X) #9993 | 10 x 600 µl |
Product Information
Product Usage Information
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit.
PTMScan® Phospho-Tyrosine (P-Tyr-1000) Kit has a higher sensitivity and specificity magnetic bead version: PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) kit in 10-assay (#38572) or 3-assay (#29879) formats.
PTMScan® Phospho-Tyrosine (P-Tyr-1000) Kit has a higher sensitivity and specificity magnetic bead version: PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) kit in 10-assay (#38572) or 3-assay (#29879) formats.
Storage
Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Product Description
PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content/content.jsp?id=ptmscan-services.
Background
PhosphoScan® Technology employs phospho-residue (Tyr, Ser, Thr) motif antibodies for phosphopeptide immunoaffinity purification from cell extracts combined with LC tandem MS/MS to identify and quantify changes in phosphorylation levels (1). Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies (3,4). The Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954, developed by Cell Signaling Technology, provides an exceptionally sensitive tool for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. PTMScan® (Y
Product Citations: 32
- Lundby, A. et al. (2019) Cell 179, 543-560.e26.
- Weber, A. et al. (2019) J Virol 93.
- Jeitany, M. et al. (2018) EMBO Mol Med 10(4) e7918.
- Goel, R.K. et al. (2018) Mol Cell Proteomics 17, 925-947.
- Thompson, S.M. et al. (2018) Int J Hyperthermia 34, 812-823.
- Venkatesh, H.S. et al. (2017) Nature 549, 533-537.
- Patterson et al. (2016) Proc Natl Acad Sci U S A 113, E2094.
- Standish, A.J. et al. (2016) J Mol Biol 428, 4197-4208.
- Gu, H. et al. (2016) Mol Cell Proteomics 15, 692-702.
- Palacios-Moreno, J. et al. (2015) PLoS Comput Biol 11, e1004130.
- Stokes, M.P. et al. (2015) Proteomes 3, 160-183.
- van der Mijn, J.C. et al. (2015) J Proteomics 127, 259-63.
- Verma, R. et al. (2014) J Exp Med 211, 1715-22.
- Paardekooper Overman, J. et al. (2014) Mol Cell Biol 34, 2874-89.
- Yoshida, T. et al. (2014) Clin Cancer Res 20, 4059-4074.
- Gu, T.L. et al. (2011) PLoS One 6, e15640.
- Pighi, C. et al. (2011) Cell Oncol (Dordr) 34, 141-53.
- Brave, S.R. et al. (2011) Mol Cancer Ther 10, 861-73.
- Andersen, J.N. et al. (2010) Sci Transl Med 2, 43ra55.
- Knowlton, M.L. et al. (2010) PLoS One 5, e13587.
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Gu, T.L. et al. (2007) Leukemia 21, 563-6.
- Gu, T.L. et al. (2007) Blood 110, 323-33.
- Mercher, T. et al. (2006) Blood 108, 2770-9.
- Griswold, I.J. et al. (2006) Mol Cell Biol 26, 6082-93.
- Gu, T.L. et al. (2006) Blood 108, 4202-4.
- Goss, V.L. et al. (2006) Blood 107, 4888-97.
- Zhang, Y. et al. (2005) Mol Cell Proteomics 4, 1240-50.
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
- Chua, X.Y. et al. (2020) Mol Cell Proteomics , .
- van Alphen, C. et al. (2020) Mol Cell Proteomics , .
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
AcetylScan is a registered trademark of Cell Signaling Technology, Inc.
MethylScan is a registered trademark of Cell Signaling Technology, Inc.
UbiScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.