PathScan® Sandwich ELISA Control Phospho Cell Extract I #7988
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Storage
Supplied as lyophilized product. Keep at -20°C for short term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles.
Product Description
Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.
Background
PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts I can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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