Name: PathScan ® Total VEGFR-2 Sandwich ELISA Antibody Pair
Price: 512.00 USD
Availability: InStock

Supporting Data

REACTIVITY

H

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human

Product Information

Storage

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Protocol

Product Description

CST's PathScan^® Total VEGFR-2 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan^® Total VEGFR-2 Sandwich ELISA Kit #7340. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The VEGFR-2 capture antibody is coated on a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a VEGFR-2 detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total VEGFR-2 protein.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan^® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

Database Links

UniProt ID: P35968

Entrez-Gene Id: 3791

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Total VEGFR-2 Sandwich ELISA Antibody Pair #7825