渲染靶标:SSR
Render Timestamp: 2025-04-02T04:23:05.931Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:17:30.503
Product last modified at: 2025-01-01T09:02:21.269Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

α-Tubulin (11H10) Rabbit mAb (BSA and Azide Free) #77763

Filter:
  • WB
  • IHC
  • IF
  • F
  • ELISA
Western Blotting Image 1: α-Tubulin (11H10) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from C6, COS-7, NIH/3T3 and HeLa cells, using α-Tubulin (11H10) Rabbit mAb. Data were generated using the standard formulation of this product.

To Purchase # 77763

Supporting Data

REACTIVITY H M R Mk Dm Z B Pg
SENSITIVITY Endogenous
MW (kDa) 52
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Dm-D. melanogaster 
  • Z-Zebrafish 
  • B-Bovine 
  • Pg-Pig 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #2125. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #2125

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

α-Tubulin (11H10) Rabbit mAb (BSA and Azide Free) detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish, Bovine, Pig

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin protein.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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