渲染靶标:SSR
Render Timestamp:
4/4/2025, 11:22:50 PM EDT
4/5/2025, 3:22:50 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:11:00.266
Product last modified at: 2025-03-21T11:30:11.356Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD133 (D2V8Q) XP® Rabbit mAb #64326

Filter:
  • WB
  • IHC
  • IF
Western Blotting Image 1: CD133 (D2V8Q) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using CD133 (D2V8Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 64326

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 133
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:200 - 1:800
Immunohistochemistry (Paraffin) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #51917.

Protocol

Specificity / Sensitivity

CD133 (D2V8Q) XP® Rabbit mAb recognizes endogenous levels of total CD133 protein. This antibody is not sensitive to the glycosylation status of CD133.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312.

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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