Neutrophil Elastase (F4H3X) Rabbit mAb (BSA and Azide Free) #61891
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to human neutrophil elastase protein.
Background
Neutrophil elastase is a hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, ELA2, have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7).
- Belaaouaj, A. et al. (2000) Science 289, 1185-8.
- Tkalcevic, J. et al. (2000) Immunity 12, 201-10.
- Gullberg, U. et al. (1999) J Immunol Methods 232, 201-10.
- Takahashi, H. et al. (1988) J Biol Chem 263, 2543-7.
- Adkison, A.M. et al. (2002) J Clin Invest 109, 363-71.
- Horwitz, M. et al. (1999) Nat Genet 23, 433-6.
- Bellanné-Chantelot, C. et al. (2004) Blood 103, 4119-25.
限制使用
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