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Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:08:33.816
Product last modified at: 2025-03-07T08:00:57.087Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Insulin Receptor β (L55B10) Mouse mAb #3020

Filter:
  • WB
  • IP
Western Blotting Image 1: Insulin Receptor β (L55B10) Mouse mAb
Western blot analysis of cell lysates from CHO and CHO/IR transfected with insulin receptor expression cDNA, using Insulin Receptor β (L55B10) Mouse mAb.

To Purchase # 3020

Cat. #

Size

Price

Inventory

3020T
20 µl
¥1,370

库存紧张

3020S
100 µl
¥4,550

库存紧张

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 95
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #60076.

Protocol

Specificity / Sensitivity

Insulin Receptor beta (L55B10) Mouse mAb detects endogenous levels of total insulin receptor β.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human insulin receptor β carboxy-terminal fragments.

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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