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Render Timestamp: 2024-10-24T19:37:12.121Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-01 15:30:43.598
Product last modified at: 2024-10-15T11:16:07.108Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. GABARAPL1 (D5R9Y) XP® Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GABARAPL1 (D5R9Y) XP® Rabbit mAb #26632

Filter:
  • WB
  • IF
  • F

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 14, 16
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #37737.

    Protocol

    Specificity / Sensitivity

    GABARAPL1 (D5R9Y) XP® Rabbit mAb recognizes endogenous levels of total GABARAPL1 protein. This antibody does not cross react with other GABARAP family members.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GABARAPL1 protein.

    Background

    GABAA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11).
    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) appears to be more highly expressed in the CNS as compared to other family members (12-14). Expression of GABARAPL1 is associated with prognosis of some cancers, including hepatocellular and breast cancer (15,16). Inhibition of GABARAPL1 expression in breast cancer cells attenuates autophagic flux, results in metabolic changes, and leads to cancer promoting activities (17).
    1. Wang, H. et al. (1999) Nature 397, 69-72.
    2. Shpilka, T. et al. (2011) Genome Biol 12, 226.
    3. Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
    4. Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24.
    5. Tanida, I. et al. (2004) J Biol Chem 279, 36268-76.
    6. Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50.
    7. Tanida, I. et al. (2001) J Biol Chem 276, 1701-6.
    8. Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
    9. Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505.
    10. Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21.
    11. Vernier-Magnin, S. et al. (2001) Biochem Biophys Res Commun 284, 118-25.
    12. Nemos, C. et al. (2003) Brain Res Mol Brain Res 119, 216-9.
    13. Wang, Y. et al. (2006) Neuroscience 140, 1265-76.
    14. Le Grand, J.N. et al. (2013) PLoS One 8, e63133.
    15. Liu, C. et al. (2014) Oncol Rep 31, 2043-8.
    16. Berthier, A. et al. (2010) Br J Cancer 102, 1024-31.
    17. Boyer-Guittaut, M. et al. (2014) Autophagy 10, 986-1003.

    Database Links

    UniProt ID: Q9H0R8


    Entrez-Gene Id: 23710


    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    For Research Use Only. Not For Use In Diagnostic Procedures.
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