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β-Gal (14B7) Mouse mAb #2372

Filter:
  • WB
  • IP
  • IF
  • F
Western Blotting Image 1: β-Gal (14B7) Mouse mAb
Western blot analysis of extracts from COS-7 cells, mock transfected (lane 1) or transiently transfected with a construct expressing beta-galactosidase (lane 2) using β-Gal (14B7) Mouse mAb.

To Purchase # 2372

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:3200
Flow Cytometry (Fixed/Permeabilized) 1:7000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Beta-Gal 14B7 Mouse mAb detects transfected beta-galactosidase fusion proteins.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues.
For Research Use Only. Not For Use In Diagnostic Procedures.
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