Product Includes	Volume (with Count)	Solution Color
FastScan™ ELISA Microwell Strip Plate, 96 Well #53257	1 x 96 tests
Rb Mouse Capture mAb #73419	1 x 1 ea	Green (Lyophilized)
Rb Rabbit HRP-linked mAb #96826	1 x 1 ea	Red (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent #16076	1 x 3 ml	Green
FastScan™ ELISA HRP Antibody Diluent #28120	1 x 3 ml
TMB Substrate #7004	1 x 11 ml
STOP Solution #7002	1 x 11 ml
Sealing Tape #54503	1 x 1 ea
ELISA Wash Buffer (20X) #9801	1 x 25 ml
FastScan™ ELISA Cell Extraction Buffer (5X) #69905	1 x 10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243	1 x 1 ml
FastScan™ ELISA Kit #23595 Positive Control Type 1 #23348	1 x 1 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The FastScan™ Total Rb ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Rb. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with Rb in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of Rb.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The FastScan™ Total Rb ELISA Kit detects endogenous levels of Rb as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Monkey

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

Pathways

Explore pathways related to this product.

Database Links

UniProt ID: P06400

Entrez-Gene Id: 5925

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

FastScan™ Total Rb ELISA Kit #23595

Important Ordering Details

Supporting Data

Application Key:

Species Cross-Reactivity Key: