R Recombinant
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ICOS (E5F7E) Rabbit mAb (BSA and Azide Free) #18670
Filter:
- WB
- IHC
- IF
Western blot analysis of extracts from various cells and tissues using ICOS (E5F7E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from mouse spleens or human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) (20 ng/ml) or Human Interleukin-2 (hIL-2) #8907 (20 ng/ml). Data were generated using the standard formulation of this product.
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22-35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
This product is the carrier free version of product #67223. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #67223
For standard formulation of this product see product #67223
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Protocol
Specificity / Sensitivity
ICOS (E5F7E) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total mouse ICOS protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val198 of mouse ICOS protein.
Background
ICOS (Inducible Co-Stimulator, CD278) is a member of the CD28 family that regulates T cell activity and immune responses (1). The ICOS protein contains an extracellular IgV-like domain, a transmembrane domain, and an intracellular domain with a YMFM motif (1-2). ICOS is primarily expressed on activated CD4+ and CD8+ T cells (1). Upon binding to its ligand, ICOS potentiates the T cell response to antigen through activation of the PI3K signaling pathway (3). In addition to enhancing T cell activation and proliferation, ICOS plays an important role in the regulation of T follicular helper cells (4). Research studies suggest that ICOS is a potential therapeutic target, and could serve as a prognostic biomarker for neoplastic therapy involving CTLA-4 blockade (5-7).
- Hutloff, A. et al. (1999) Nature 397, 263-6.
- Coyle, A.J. et al. (2000) Immunity 13, 95-105.
- Fos, C. et al. (2008) J Immunol 181, 1969-77.
- Hutloff, A. (2015) Oncotarget 6, 21785-6.
- Kyi, C. and Postow, M.A. (2014) FEBS Lett 588, 368-76.
- Fan, X. et al. (2014) J Exp Med 211, 715-25.
- Ng Tang, D. et al. (2013) Cancer Immunol Res 1, 229-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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