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Phospho-Androgen Receptor (Ser258) (E4L1I) Rabbit mAb #16969
Filter:
- WB
- IP
- ChIP
Western blot analysis of extracts from LNCaP cells grown in phenol red free media and 5% charcoal stripped FBS for 3 d then either untreated (-), dihydrotestosterone (DHT)-treated (+), or DHT-treated and subsequently treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-Androgen Receptor (Ser258) (E4L1I) Rabbit mAb (upper) or Androgen Receptor (D6F11) XP® Rabbit mAb #5153 (lower).
This product is discontinued
Inquiry Info. # 16969
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #87419.
For a carrier free (BSA and azide free) version of this product see product #87419.
Protocol
Specificity / Sensitivity
Phospho-Androgen Receptor (Ser258) (E4L1I) Rabbit mAb recognizes endogenous levels of androgen receptor protein only when phosphorylated at Ser258.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser258 of human androgen receptor protein.
Background
Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
Upon ligand binding, AR becomes phosphorylated on many residues, including Ser258 (4).
Upon ligand binding, AR becomes phosphorylated on many residues, including Ser258 (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
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