Phospho-NuMA (Thr2055) Antibody #14951
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 238 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-NuMA (Thr2055) Antibody recognizes endogenous levels of NuMA protein only when phosphorylated at Thr2055.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr2055 of human NuMA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).
Phosphorylation at Thr2055 by CDK1 is required for spindle pole association of NuMA at the onset of mitosis. Dephosphorylation by PPP2CA leads to enhancement of NuMA at the cell cortex in anaphase and proper cell-cycle progression (5,6).
Phosphorylation at Thr2055 by CDK1 is required for spindle pole association of NuMA at the onset of mitosis. Dephosphorylation by PPP2CA leads to enhancement of NuMA at the cell cortex in anaphase and proper cell-cycle progression (5,6).
- Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
- Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
- Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
- Matsuoka, S. et al. (2007) Science 316, 1160-6.
- Kotak, S. et al. (2013) EMBO J 32, 2517-29.
- Seldin, L. et al. (2013) Mol Biol Cell 24, 3651-62.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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