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Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb (PE Conjugate) #12602
Filter:
- F
Inquiry Info. # 12602
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb #9496.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb (PE Conjugate) recognizes human caspase-8 when cleaved at Asp374 (isoform B, Asp391 on isoform A). This antibody detects cleavage products containing the pro-domain with the p18 subunit, as well as the p18 subunit alone.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp374 of human caspase-8 protein.
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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