R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb #12316
Filter:
- WB
Western blot analysis of extracts from 293T, PANC-1, and HCT 116 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb (upper), Scribble Antibody #4475 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 240 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb recognizes endogenous levels of scribble protein only when phosphorylated at Ser1220.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1220 of human scribble protein.
Background
Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8).
The phosphorylation state of Scribble has been shown to be functionally important, in part by regulating subcellular localization (9). Mass spectrometry studies have identified phosphorylation at Ser1220 as a frequent modification in a variety of cell and tissue types (10-13). The functional significance of this modification remains to be elucidated.
The phosphorylation state of Scribble has been shown to be functionally important, in part by regulating subcellular localization (9). Mass spectrometry studies have identified phosphorylation at Ser1220 as a frequent modification in a variety of cell and tissue types (10-13). The functional significance of this modification remains to be elucidated.
- Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
- Bilder, D. et al. (2000) Science 289, 113-6.
- Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
- Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
- Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
- Navarro, C. et al. (2005) Oncogene 24, 4330-9.
- Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
- Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.
- Yoshihara, K. et al. (2011) Exp Cell Res 317, 413-22.
- Olsen, J.V. et al. (2010) Sci Signal 3, ra3.
- Han, G. et al. (2010) Electrophoresis 31, 1080-9.
- Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13.
- Wang, Y.T. et al. (2010) J Proteome Res 9, 5582-97.
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