Render Target: SSR
Render Timestamp: 2025-01-10T02:51:40.800Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2025-01-08 16:43:17.915
Product last modified at: 2025-01-01T09:03:04.615Z
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PDP - Template Name: Oligo Antibody Pair
PDP - Template ID: *******46423d7

PD-1 (Intracellular Domain) (D4W2J) & CO-0008-647 SignalStar Oligo-Antibody Pair #56837

Filter:
  • IHC
Immunohistochemistry Image 1: PD-1 (Intracellular Domain) (D4W2J) & CO-0008-647 SignalStar™ Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using PD-1 (Intracellular Domain) (D4W2J) & CO-0008-594 SignalStar™ Oligo-Antibody Pair #35347 (yellow), Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (red), PD-L1 (E1L3N®) & CO-0005-488 SignalStar™ Oligo-Antibody Pair #85646 (green), Granzyme B (D6E9W) & CO-0009-594 SignalStar™ Oligo-Antibody Pair #15194 (orange), TIGIT (E5Y1W) & CO-0002-647 SignalStar™ Oligo-Antibody Pair #18288 (cyan), CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair #36775 (pink), CD8α (D8A8Y) & CO-0004-750 SignalStar™ Oligo-Antibody Pair #62750 (white), CD3ε (D7A6E™) & CO-0001-750 SignalStar™ Oligo-Antibody Pair #51754 (magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 56837

This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
PD-1 (Intracellular Domain) (D4W2J) XP® Rabbit mAb (SignalStar™ Conjugate 0008) #7272750 µlHRabbit IgG
Complementary Oligo (CO-0008-647) #7704722 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

PD-1 (Intracellular Domain) (D4W2J) XP® Rabbit mAb (SignalStar™ Conjugate 0008) recognizes endogenous levels of total PD-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein.

Background

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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