Single-cell RNA sequencing (scRNA-seq) is a powerful technique for studying the molecular heterogeneity of cells. However, scRNA-seq does not provide information about protein expression, which is essential for understanding the functional state of cells. In this video, we introduce InTraSeq Single Cell Analysis, a novel technology developed by Cell Signaling Technology (CST). InTraSeq (Intracellular Protein and Transcriptomic Sequencing) enables the concurrent measurement of RNA, surface markers, cytoplasmic proteins, and nuclear proteins within individual cells. By leveraging renowned CST® antibodies, InTraSeq Single Cell Analysis also enables the study of signaling pathways at single-cell resolution. Developed and validated by CST, InTraSeq 3’ technology uses the 10x Genomics Chromium Single Cell 3’ Reagent Kits with Feature Barcoding technology.