DRAQ5® #4084
Filter:
- IF
- F
Product Information
Product Usage Information
Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.
Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.
Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.
Storage
Store at 4°C protected from light.
Background
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).
Product Citations: 8
- Galitovskiy, V. et al. (2011) J Immunol 187, 2677-87.
- Talchai, C. et al. (2012) Cell 150, 1223-34.
- Talchai, C. et al. (2012) Nat Genet 44, 406-12, S1.
- Rozelle, D.K. et al. (2014) Mol Cell Biol 34, 2003-16.
- Huo, Y. and Macara, I.G. (2014) Nat Cell Biol 16, 529-37.
- Sun, X. et al. (2015) Oncotarget 6, 32944-54.
- Boucrot, E. et al. (2015) Nature 517, 460-5.
- O'Hagan-Wong, K. et al. (2016) Oncotarget 7, 13285-96.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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