SignalSilence® HSP27 siRNA I #6356
Supporting Data
| REACTIVITY | H |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
CST recommends transfection with 100nM HSP27 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Storage
Product Description
Background
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
Small interfering RNA (siRNA) has been used to specifically silence HSP27 expression in HeLa cells (7).
- Stetler, R.A. et al. (2009) Curr Mol Med 9, 863-72.
- Landry, J. et al. (1992) J Biol Chem 267, 794-803.
- Rouse, J. et al. (1994) Cell 78, 1027-37.
- Rogalla, T. et al. (1999) J Biol Chem 274, 18947-56.
- Lavoie, J.N. et al. (1993) J Biol Chem 268, 24210-4.
- Rousseau, S. et al. (1997) Oncogene 15, 2169-77.
- Park, K. et al. (2003) J. Biol. Chem. 278, 35272-35278.
限制使用
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