Anti-mouse IgG (H+L), F(ab')2 Fragment (DyLight® 488 Conjugate) #4316
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Inquiry Info. # 4316
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Supporting Data
REACTIVITY | |
SENSITIVITY | |
MW (kDa) | |
Source/Isotype | Goat |
Product Information
Product Description
Product Usage Information
For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000. See protocol for more details.
Storage
Specificity / Sensitivity
Source / Purification
The Anti-mouse IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with mouse IgG whole molecules. The Anti-mouse IgG (H+L),
F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion and chromatographic separation. The F(ab')2 Fragments were conjugated to DyLight® 488 under optimal conditions with an F/P ratio of 2-6.
Background
Fluorescent Anti-mouse IgG (H+L), F(ab')2 Fragment is ideal for flow cytometry and immunofluorescence. This secondary antibody will detect mouse antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.
限制使用
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