Revision 1

#79030

Store at -20C

TIF1α/TRIM24 (E9T3N) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
140, 160, 180

Source/Isotype:
Rabbit IgG

UniProt ID:
#O15164

Entrez-Gene Id:
8805

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

TIF1α/TRIM24 (E9T3N) Rabbit mAb recognizes endogenous levels of total TIF1α/TRIM24 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein.

Background

TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17).

Background References

  1. Le Douarin, B. et al. (1995) EMBO J 14, 2020-33.
  2. Le Douarin, B. et al. (1996) EMBO J 15, 6701-15.
  3. Heery, D.M. et al. (1997) Nature 387, 733-6.
  4. Le Douarin, B. et al. (1998) J Steroid Biochem Mol Biol 65, 43-50.
  5. Khetchoumian, K. et al. (2008) Cell Cycle 7, 3647-52.
  6. Ignat, M. et al. (2008) Proc Natl Acad Sci U S A 105, 2598-603.
  7. Khetchoumian, K. et al. (2007) Nat Genet 39, 1500-6.
  8. Tsai, W.W. et al. (2010) Nature 468, 927-32.
  9. Kikuchi, M. et al. (2009) Biochim Biophys Acta 1793, 1828-36.
  10. Allton, K. et al. (2009) Proc Natl Acad Sci U S A 106, 11612-6.
  11. Tai, E. and Benchimol, S. (2009) Proc Natl Acad Sci U S A 106, 11431-2.
  12. Jain, A.K. et al. (2014) Mol Cell Biol 34, 2695-709.
  13. Li, H. et al. (2012) PLoS One 7, e37657.
  14. Zhang, L.H. et al. (2015) Oncogene 34, 600-10.
  15. Fang, Z. et al. (2017) Oncol Lett 13, 1797-1806.
  16. Lv, D. et al. (2017) Nat Commun 8, 1454.
  17. Zhu, Y. et al. (2018) Exp Cell Res 367, 274-281.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.