TCF4/ITF2 (E3X2Y) Rabbit mAb recognizes endogenous levels of total TCF4/ITF2 protein. This antibody is not recommended for immunohistochemical analysis of human tissues. TCF4/ITF2 (E3X2Y) Rabbit mAb is predicted to detect all 16 isoforms of human TCF4/ITF2 protein produced by alternative splicing described on Uniprot (P15884-1 through P15884-16).

Bovine, Dog, Pig, Horse, Rabbit

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein.

Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14).