TCF4/ITF2 (E3X2Y) Rabbit mAb #46008
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62-82 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:1500 - 1:6000 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:3200 - 1:6400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein.
Background
Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14).
- Murre, C. et al. (1994) Biochim Biophys Acta 1218, 129-35.
- Furumura, M. et al. (2001) J Biol Chem 276, 28147-54.
- Sepp, M. et al. (2011) PLoS One 6, e22138.
- Flora, A. et al. (2007) Proc Natl Acad Sci USA 104, 15382-7.
- Brockschmidt, A. et al. (2007) Hum Mol Genet 16, 1488-94.
- Rannals, M.D. and Maher, B.J. (2017) Curr Genet Med Rep 5, 1-7.
- Stefansson, H. et al. (2009) Nature 460, 744-7.
- Mesman, S. et al. (2020) Mol Cell Neurosci 106, 103502.
- Papes, F. et al. (2022) Nat Commun 13, 2387.
- Nagasawa, M. et al. (2008) Eur J Immunol 38, 2389-400.
- Cisse, B. et al. (2008) Cell 135, 37-48.
- Dewald, H.K. et al. (2020) Viruses 12, 162.
- Pernía, O. et al. (2020) Cancers (Basel) 12, 786.
- Pozniak, J. et al. (2024) Cell 187, 166-183.e25.
限制使用
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