Revision 1

#25792

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
ChIP

Reactivity:
H

Sensitivity:
Endogenous

Source/Isotype:
Rabbit IgG

UniProt ID:
#P35711

Entrez-Gene Id:
6660

Product Usage Information

For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Chromatin IP 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SOX5 (E8S4A) Rabbit mAb detects endogenous levels of SOX5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SOX5 protein.

Background

SOX5 is a member of the SOX (SRY-related HMG-box) family of transcription factors. It is involved in the regulation of embryonic development, and specifically essential for driving chondrogenesis (1). In recent years, it has become apparent that SOX5 plays a significant role in the progression of various tumor types, such as breast cancer, glioma, hepatocellular carcinoma, lung cancer, and others (2-5). Upregulation of SOX5 in these tumor types has been reported to drive metastasis through activation of epithelial-mesenchymal transition (EMT) (2-5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

ChIP: Chromatin IP

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated HDLM-2 cells and either SOX5 (E8S4A) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human RBM20 promoter primers, human SCUBE2 intron 7 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin Immunoprecipitation Image 1: SOX5 (E8S4A) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.