Revision 1

#29599

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
30-40

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q6DKI7

Entrez-Gene Id:
79037

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

PVRIG (E9X9B) Rabbit mAb recognizes endogenous levels of total PVRIG protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val325 of human PVRIG protein.

Background

Poliovirus Receptor-Related Immunoglobulin Domain-Containing Protein (PVRIG; also known as CD112 receptor) is a multiple transmembrane protein expressed on the surface of T cells and NK cells, predominantly on memory and effector CD8+ T cells. PVRIG expression is upregulated after in vitro T cell activation with anti-CD3 and anti-CD28 antibodies. PVRIG competes with DNAM-1/CD226 for interaction with the receptor ligand Nectin-2. Upon ligation of PVRIG and Nectin-2, T cell proliferation is inhibited, suggesting PVRIG is a co-inhibitory receptor that dampens T cell functions (1).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various human cells using PVRIG (E9X9B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: PVRIG (E9X9B) Rabbit mAb
Immunoprecipitation of PVRIG protein from CCRF-CEM cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PVRIG (E9X9B) Rabbit mAb. Western blot analysis was performed using PVRIG (E9X9B) Rabbit mAb.
Immunoprecipitation Image 1: PVRIG (E9X9B) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.