Revision 1
#19472
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
79
Source/Isotype:
Rabbit IgG
UniProt ID:
#O75909
Entrez-Gene Id:
8812
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Due to its importance in maintaining genome stability through regulation of the DDR, the cyclin K/CDK12 complex is an emerging therapeutic target in human cancer (4,5). Targeted protein degradation via molecular glues and PROTACs have been employed to specifically degrade cyclin K/CDK12 as a means of targeting the DDR in human cancer (6-9).
Background References
- Lei, T. et al. (2018) Nat Commun 9, 1876.
- Blazek, D. (2012) Cell Cycle 11, 1049-50.
- Hoshii, T. et al. (2018) Cell 172, 1007-1021.e17.
- Emadi, F. et al. (2020) Drug Discov Today 25, 2257-2267.
- Liu, H. et al. (2021) Cancer Res 81, 18-26.
- Lv, L. et al. (2020) Elife 9, e59994. doi: 10.7554/eLife.59994.
- Sun, R. et al. (2022) Proc Natl Acad Sci USA 119, e2205509119.
- Niu, T. et al. (2022) Eur J Med Chem 228, 114012.
- Sano, O. et al. (2023) Biochem Biophys Res Commun 676, 6-12.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
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Revision 1
#19472
Cyclin K (E7F4N) Rabbit mAb
