Cyclin K (E7F4N) Rabbit mAb #19472
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 79 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human cyclin K protein.
Background
Cyclin K is a positive regulator of transcriptional cyclin-dependent kinases (CDKs) CDK12 and CDK13. The cyclin K/CDK12 complex regulates the formation of the pre-replicative complex during G1 phase of the cell cycle (1), as well as the expression of key proteins in the DNA Damage Response (DDR) (2). Regulation of DDR-related gene expression involves cyclin K interaction with the histone methyltransferase SETD1A (3).
Due to its importance in maintaining genome stability through regulation of the DDR, the cyclin K/CDK12 complex is an emerging therapeutic target in human cancer (4,5). Targeted protein degradation via molecular glues and PROTACs have been employed to specifically degrade cyclin K/CDK12 as a means of targeting the DDR in human cancer (6-9).
- Lei, T. et al. (2018) Nat Commun 9, 1876.
- Blazek, D. (2012) Cell Cycle 11, 1049-50.
- Hoshii, T. et al. (2018) Cell 172, 1007-1021.e17.
- Emadi, F. et al. (2020) Drug Discov Today 25, 2257-2267.
- Liu, H. et al. (2021) Cancer Res 81, 18-26.
- Lv, L. et al. (2020) Elife 9, e59994. doi: 10.7554/eLife.59994.
- Sun, R. et al. (2022) Proc Natl Acad Sci USA 119, e2205509119.
- Niu, T. et al. (2022) Eur J Med Chem 228, 114012.
- Sano, O. et al. (2023) Biochem Biophys Res Commun 676, 6-12.
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