Revision 6

#64326

Store at -20C

CD133 (D2V8Q) XP® Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-Bond, IHC-P, IF-IC

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
133

Source/Isotype:
Rabbit IgG

UniProt ID:
#O43490

Entrez-Gene Id:
8842

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:200 - 1:800
Immunohistochemistry (Paraffin) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #51917.

Specificity/Sensitivity

CD133 (D2V8Q) XP® Rabbit mAb recognizes endogenous levels of total CD133 protein. This antibody is not sensitive to the glycosylation status of CD133.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312.

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-Bond: IHC Leica Bond IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

#64326

CD133 (D2V8Q) XP® Rabbit mAb

CST Logo
Western blot analysis of extracts from various cell lines using CD133 (D2V8Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Western blot analysis of extracts from WERI-Rb1 cells, untreated (-) or treated with PNGase F (+), using CD133 (D2V8Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using CD133 (D2V8Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Immunohistochemistry Image 1: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

#64326

CD133 (D2V8Q) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD133 (D2V8Q) XP® Rabbit mAb.
Immunohistochemistry Image 1: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD133 (D2V8Q) XP® Rabbit mAb.
Immunohistochemistry Image 2: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human kidney using CD133 (D2V8Q) XP® Rabbit mAb.
Immunohistochemistry Image 3: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

#64326

CD133 (D2V8Q) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CD133 (D2V8Q) XP® Rabbit mAb.
Immunohistochemistry Image 4: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded Caco-2 cell pellet (left, positive) or HeLa cell pellet (right, negative) using CD133 (D2V8Q) XP® Rabbit mAb.
Immunohistochemistry Image 5: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of WERI-Rb-1 cells (left, high-expressing) and MCF7 cells (right, low-expressing) using CD133 (D2V8Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunofluorescence Image 1: CD133 (D2V8Q) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.