Revision 3

#28299

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877-678-TECH (8324)

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For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H Mk

Sensitivity:
Endogenous

MW (kDa):
190, 220

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q6VMQ6

Entrez-Gene Id:
55729

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Specificity/Sensitivity

ATF7IP (F8C9I) Rabbit mAb recognizes endogenous levels of total ATF7IP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ATF7IP protein.

Background

Activating transcription factor 7-interacting protein (ATF7IP), also known as MCAF1 or AM, is a repressive chromatin factor that binds to SETDB1, enhancing its ability to catalyze histone H3 lysine 9 trimethylation (1-3). ATF7IP has a SUMO binding domain, which interacts with sumoylated MBD1 to recruit the SETDB1/ATF7IP heterodimer to sites of heterochromatin (4). Loss of ATF7IP results in degradation of SETDB1 protein and a global loss of H3K9me3 (5). ATF7IP can localize to PML bodies in a SUMO-dependent manner, causing reduced histone mRNA and protein and cellular senescence (6). ATF7IP has also been implicated in cancer, where it interacts with SP1 to regulate telomerase. It can also contribute to immune cell function by inhibiting the IL-2 and IL-7R genes (7-9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

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Western blot analysis of extracts from various cell lines using ATF7IP (F8C9I) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Low expression of ATF7IP protein in HuH-6 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: ATF7IP (F8C9I) Rabbit mAb
Immunoprecipitation of ATF7IP protein from KYSE-70 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ATF7IP (F8C9I) Rabbit mAb. Western blot analysis was performed using ATF7IP (F8C9I) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: ATF7IP (F8C9I) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.